evaluation of cisplatin and cisplatin-loaded magnetic iron oxide nanoparticles on bcl2 and bax genes in the breast cancer t47d cell line

نویسندگان

mohammad javad mokhtari

zhilla hoseineian

fatemeh koohpeima

azim akbarzadeh

mehrdad hashemi

چکیده

objective: breast cancer is the second leading cause of cancer death in women. cisplatin is a traditional cancer drug commonly used in chemotherapy for killing cancer cells. modulation at the mrna levels of apoptotic related genes often correlate with the sensitivity of various types of cancer cells to chemotherapeutic agents. nanoparticulate drug delivery systems are being developed to effectively deliver smaller doses of chemotherapeutic agents and control drug distribution in the body. in this study, we evaluate the expressions of bcl2 and bax genes in t47d treated with cisplatin and cisplatin nanoparticles, which can result in a new approach to breast cancer therapy. methods: in this study, we treated t47d cells with different concentrations of cisplatin and cisplatin nanoparticles at 48 h. the ic50 was determined. we extracted rna by using rnx solution, after which cdna was synthesized. the precise primers for the bcl2, bax and tbp genes were designed by specific software. the quantity of bcl2 and bax gene expression compared to tbp gene (reference gene) was analyzed using real-time pcr.  results: bcl2 and bax gene expression levels in t47d cells treated by cisplatin were 0.7 (bcl2) and 1.48 (bax); in t47d cells treated with cisplatin-loaded nanoparticles, the gene expressions were 0.03 (bcl2) and 2.41 (bax). conclusion: in this study, the results have shown that cisplatin-loaded nanoparticles are effective anticancer agents. cisplatin nanoparticles induce apoptosis in human breast cancer cell lines. we have shown that cisplatin nanoparticles strongly increased cytotoxicity in comparison to the free drug in the t47d cell line.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Induction of Apoptosis and Non-Apoptosis in Human Breast Cancer Cell Line (MCF-7) by Cisplatin and Caffeine

Background: Molecular targeted therapy by different cell death inducers are recently considered in cancer therapy. The aim of this study was to compare the effect of cisplatin and inositol trisphosphate kinase inhibitor (caffeine) on human breast cancer cell line (MCF-7). The pattern of cell death in MCF-7 cells following the exposure to cisplatin and caffeine in individual and combination form...

متن کامل

evaluation of cellular toxicity for cisplatin, arsenic and acetaminophen in the cancer and normal cell line

introduction: cell culture is a process in which the cells ware isolated from original tissue, dispersed in liquid media and then placed in culture plate where the cells adhere together and propagate. today, this method is used for assessment of cell toxicity, its mechanisms and effect of different compounds on intracellular components. methods: clonogenic assay was used for assessment of cell ...

متن کامل

The Effects of Synthesized Superparamagnetic Iron Oxide Nanoparticles and Electromagnetic Field on Cell Death of MCF-7 Breast Cancer Cell Line

Introduction: Iron oxide nanoparticles, owing to their very small size and superparamagnetic properties, have been considered a potential candidate for several medical applications such as magnetic cell separation, magnetic resonance imaging (MRI), magnetic targeted drug delivery magnetichyperthermia. The present study aimed to synthesize and evaluate the characteristics of super...

متن کامل

The Effects of Synthesized Superparamagnetic Iron Oxide Nanoparticles and Electromagnetic Field on Cell Death of MCF-7 Breast Cancer Cell Line

Introduction: Iron oxide nanoparticles, owing to their very small size and superparamagnetic properties, have been considered a potential candidate for several medical applications such as magnetic cell separation, magnetic resonance imaging (MRI), magnetic targeted drug delivery magnetichyperthermia. The present study aimed to synthesize and evaluate the characteristics of super...

متن کامل

the study of aaag repeat polymorphism in promoter of errg gene and its association with the risk of breast cancer in isfahan region

چکیده: سرطان پستان دومین عامل مرگ مرتبط با سرطان در خانم ها است. از آنجا که سرطان پستان یک تومور وابسته به هورمون است، می تواند توسط وضعیت هورمون های استروئیدی شامل استروژن و پروژسترون تنظیم شود. استروژن نقش مهمی در توسعه و پیشرفت سرطان پستان ایفا می کند و تاثیر خود را روی بیان ژن های هدف از طریق گیرنده های استروژن اعمال می کند. اما گروه دیگری از گیرنده های هسته ای به نام گیرنده های مرتبط به ا...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
modares journal of medical sciences: pathobiology

ناشر: tarbiat modares university

ISSN 1562-9554

دوره 15

شماره 4 2012

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023